PNAS2003年关于GEOSMIN合成基因的文章,引发了2004年强哥及自己的一些想法。
1,探求蓝藻中相关的基因(强哥)
2,基因敲除---合成途经(我)
3,FPPS/GPPS基因---引物合成---特异专一条带的得到----部分序列----适用于至少2种以上的产MIB,GEOSMIN的蓝藻(我,2004)
+++++++++
4,基因walker kit的发现,产生用walker法得到FPPS/GPPS基因全长及相邻序列的想法(05/2005)
5,检查Streptomyces coelicolor genome,发现SCO6073 (cyclase)与FPPS/GPPS/。。并不是在相近的区,
Smallest
Sum
High Probability
Sequences producing High-scoring Segment Pairs: Score P(N) N
SCD20.01 SCO4583 putative transferase 5004916:5005926 for... 241 9.0e-25 2
2SC7G11.12 gtr SCO5250 polyprenyl synthetase 5711116:5712... 242 6.1e-22 1
crtB SCO0185 putative geranylgeranyl pyrophosphate syntha... 198 3.1e-15 1
SC6A5.12 SCO6763 putative polyprenyl synthatase 7519463:7... 190 2.6e-14 1
SCF73.12c SCO0565 putative polyprenyl synthetase 607851:6... 171 3.8e-12 1
SC8B3.02c SCO0568 putative polyprenyl synthetase 610415:6... 159 9.8e-11 1
Only 6 polyprenyl synthetase-like proteins were detected in its genome.
In 6803 genome, similar position were found,sll0506 CyanoBase undecaprenyl pyrophosphate synthetase PEDANT gi_1001342 slr0611 CyanoBase solanesyl diphosphate synthase (sds) PEDANT gi_3426161 slr0739 CyanoBase geranylgeranyl pyrophosphate synthase (crtE) CYORF geranylgeranyl pyrophosphate synthase (crtE) PEDANT gi_1651762