RACE法克隆全长基因cDNA的几个实战心得1,RNA质量尽管手册上有强调,但要多高的质量呢?其实只要用普通的琼脂糖电泳检查一下就足够:量足没有弥撒的小分子就OK了。至于是否一定要用mRNA,倒是不一定,至少对我的几个基因而言是如此。2,起始RNA的量用NANO测其A260/280的值,得其浓度,KIT手册上说是取1ug,那就取1ug。不要想着目的基因的拷贝数可能很稀少,就多加RNA。这样,其[
阅读全文]
Nature437,461(22September2005)|doi:10.1038/437461bLackoflabnotescastsdoubtonRNAresearcher'sresultsIchikoFuyuno,TokyoTopofpageAbstractJapaneseuniversityhasbeenunabletoconfirmitsprofessor'sresults.ArespectedJapanesescientistwhofailedtoproducelaboratorynotebooksconfirminghispublishedresultsnowfacesafuroreoverthecredibilityofhisfindings.On13September,theUniversityofTokyo'sSchoolofEngineeringheldapress...[
阅读全文]
EducatedWoman,TheGrad-SchoolAdventuresofMicellaPhoenixDeWhyseChapter43:WhereistheLove?BYMICELLAPHOENIXDeWHYSE
UNITEDSTATES
23SEPTEMBER2005Hellofolks,backforanotherinstallment.Well,it'sbeenquiteatripsofar,andIamabouttostartmy*fifth*yearofthisstuff,andI'vehadenough.Iwanttobeoutnow,butbeforeIcanescape,Ihavetofindaplacetoescapeto.Ifyou'vereadthelastfewinstallments,therehavebeenmusingsonwhatIco...[
阅读全文]
Bigjobsthatpaybadly
Somecareerscosttimeandmoneytotakeup.Butdon'texpectabigpaycheck.
August17,2005:10:39AMEDT
ByJeanneSahadi,CNN/Moneyseniorwriter
EnterKeyword(s):Help
EnteraCity:
SelectaState:
–AllUnitedStates–AlabamaAlaskaArizonaArkansasCaliforniaColoradoConnecticutDelawareDistrictofColumbiaFloridaGeorgiaHawaiiIdahoIllinoisIndianaIowaKansasKentuckyLouisianaMaineMaryl...[
阅读全文]
ReviewersCanHelpGetYourPaperPublishedDAVIDGRIMM
UNITEDSTATES
23SEPTEMBER2005RELATEDARTICLESRepostedfromScienceMagazine,23September2005CHICAGO,ILLINOIS--It'stheclosestmostscientistswillcometopickingtheirownjurors.Amidallthechecklists,bibliographicinformation,andfile-attachmentinstructions,themanuscriptsubmissionformsofmanyjournalsaskauthorsasimplequestion:Arethereanyindividualsyouwouldliket...[
阅读全文]
MindMatters:ManagingConflictintheLab"Conflictoverscienceandissuescanbebeneficialtoproductivityanddevelopment.Conflictoverworkingstylesandpersonalitiescanbedestructive,"saysJohnBaldoni,anAnnArbor,Michigan,managementconsultant.RELATEDARTICLESIRENES.LEVINE
UNITEDSTATES
23SEPTEMBER2005WhenpsychologistAlanLeshner,nowChiefExecutiveOfficeroftheAAASwasdirectoroftheNationalInstituteofMentalHealth(N...[
阅读全文]
TheUnitedStates,whichhasthemajorshareoftheglobalbiotechmarket,isfaringverywell,andEuropehasshownstrongsignsofrecoveryin2004.ResearchCareersintheBiotechIndustry
ELISABETHPAIN
CONTRIBUTINGEDITOR
SOUTHANDWESTEUROPE
9SEPTEMBER2005DefiningBiotechnology
Biotechnologyisallaroundus.Ifyou'renotconvinced,justopenyourfridgeorvisityourpharmacist.Yogurtsandbeer,vaccinesandantibiotics…They'...[
阅读全文]
StephenJames:"Asascientist,youmustbewillingtoworktowardmakingaproduct."Gainingnewknowledgeforitsownsakeitnotenough.BACKTOTHEFEATUREINDEX
RELATEDARTICLESALookatHealthCareBiotech
ANNEFORDE
EUROPEANEDITOR
NORTHANDEAST
9SEPTEMBER2005StephenJames'sfutureinacademiawaslookinggooduntilonedayhehadarealisation."Myideasdriedup,"hesays,"andIneededtoreinventmyself."James(picturedleft)hadbee...[
阅读全文]
拐杖
一只拐杖,雕龙画凤的,煞是好看,颇得主人的喜爱。主人赶集上店,访亲
拜友,都带着它。
也不知什么时候,这只拐杖生了虫子,有些细细的粉末从拐杖里漏了出来。
主人不理会虫子。他那只拐杖坚固得很,耐用得很,几只小小的蛀虫无损拐
杖的价值,就由它自生自灭吧。
主人这样想,就这样做。他继续拄着拐杖走来走去,忙忙[
阅读全文]
16August2005
TurningAlgaeIntoBeastsofBurden
Needtomoveaminisculeloadinamicroscopicchamber?Scientistshavediscoveredawaytomakegreenalgaecarrytwicetheirweightincargooverdistancesof20cmatspeedsofupto200micronspersecond.Thedeliveryisguidedbylightandmaysomedaybeusedinapplicationssuchasremovingtoxinsfrompollutedwater.Micromover.Chlamydomonasreinhardtiialgaecanferrypolystyrenebeadsoverlongdis...[
阅读全文]