Diaion HP-20

Diaion HP-20 is a synthetic adsorbent resin commonly used in reverse phase column chromatography. It's a non-polar polymer, specifically a styrene-divinylbenzene copolymer, known for its high porosity and ability to separate a variety of compounds. It's often used for preconcentration, desalting, and separating compounds like antibiotics, peptides, proteins, and natural products. 

 

 

 

 

 

 

 

 

Here's a more detailed explanation:

 

Key Properties and Applications:

• Reverse Phase Chromatography:

  Diaion HP-20 is a reversed-phase adsorbent, meaning it interacts with molecules based on their hydrophobicity. Non-polar compounds are retained longer on the column than polar compounds. 

   
• High Porosity:

  The resin's highly porous structure provides a large surface area for efficient adsorption of target molecules. 

   
• Applications:

  • Preconcentration: Diaion HP-20 can be used to concentrate dilute solutions of target compounds, making them easier to analyze or purify. 
     
  • Desalting: It's effective at removing salts from solutions, which is important for downstream analysis or applications. 
     
  • Separation of Biomolecules: The resin is suitable for separating and purifying various biomolecules like peptides, proteins, and antibiotics. 
     
  • Removal of Toxic Compounds: It has been shown to be effective in removing toxic compounds from fermentation broths. 
     
  • Extraction of Natural Products: Diaion HP-20 can be used to extract and purify natural pigments, herbal drugs, and other natural products. 
     

Using Diaion HP-20 in Column Chromatography:

1. 1. Column Packing:

   The resin is packed into a column, typically a glass column, as a stationary phase. 

    
2. 2. Sample Loading:

   The sample to be separated is dissolved in a suitable solvent (often water or a water/organic solvent mixture) and loaded onto the column. 

    
3. 3. Elution:

   The column is then eluted with a series of solvents of increasing polarity (e.g., water followed by a water/methanol gradient) to separate the compounds of interest. 

    
4. 4. Collection:

   Fractions are collected as the compounds elute from the column, and these fractions can be analyzed or further purified. 

    
5. 5. Regeneration:

   After use, the column can be regenerated by washing with appropriate solvents to remove any remaining adsorbed material.