Diaion HP 20 and Sephadex LH-20
文章来源: 张好古2023-07-05 19:10:18
HP-20 is a polymeric reverse phase, so it will behave pretty much like C18 in terms of a separation mechanism. Start with water, and run a gradient with methanol or ethanol as described above. One can use acids (trifluoro, formic, or acetic acid) if needed to sharpen peaks.For loading the sample, sounds like your sample is soluble in water so a liquid load is appropriate.